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control sirna targeting gfp  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology control sirna targeting gfp
    Control Sirna Targeting Gfp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control sirna targeting gfp/product/Santa Cruz Biotechnology
    Average 93 stars, based on 47 article reviews
    control sirna targeting gfp - by Bioz Stars, 2026-06
    93/100 stars

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    Image Search Results


    ILK is induced during LEC differentiation and inhibition of ILK retards expression of EMT markers α-SMA and fibronectin. A : Immunoblot of lysates (20 μg/lane) from immortalized and primary mouse LEC that grew out from dissected lens capsules (0-14 days). Lane 1, rhILK (500 ng); lane 2, immortalized LEC; lane 3, primary LEC, 0 days; lane 4, primary LEC, 7 days; lane 5, primary LEC, 14 days. Lysates were probed for ILK and α-SMA as an indicator of EMT. rhILK (lane 1) was used for protein size comparison, and a probe for GAPDH was used to normalize protein loading. B : Densitometry of protein bands from the blot shown in A . Relative expression values are normalized to those of GAPDH. C : Immunoblot of lysates (2 capsules/lane) from primary mouse LEC that grew out from dissected lens capsules (0-14 days). Lane 1, primary LEC + ILK-targeting siRNA, 7 days; lane 2, primary LEC + ILK-targeting siRNA, 14 days; lane 3, primary LEC + GFP-targeting siRNA (control), 7 days; lane 4, primary LEC + GFP-targeting siRNA. Lysates were probed for ILK to verify ILK-targeting siRNA efficiency. Fibronectin and α-SMA were monitored as indicators of EMT. An additional probe for GAPDH was used to normalize protein loading. D : Densitometry of protein bands from the blot shown in C . ILK- and GFP-siRNA-treated explants show a significant difference in protein expression after 14 days of culture (the asterisk indicates a p<0.02). Relative values are normalized to that of GAPDH.

    Journal: Molecular Vision

    Article Title: Expression of integrin-linked kinase in the murine lens is consistent with its role in epithelial-mesenchymal transition of lens epithelial cells in vitro

    doi:

    Figure Lengend Snippet: ILK is induced during LEC differentiation and inhibition of ILK retards expression of EMT markers α-SMA and fibronectin. A : Immunoblot of lysates (20 μg/lane) from immortalized and primary mouse LEC that grew out from dissected lens capsules (0-14 days). Lane 1, rhILK (500 ng); lane 2, immortalized LEC; lane 3, primary LEC, 0 days; lane 4, primary LEC, 7 days; lane 5, primary LEC, 14 days. Lysates were probed for ILK and α-SMA as an indicator of EMT. rhILK (lane 1) was used for protein size comparison, and a probe for GAPDH was used to normalize protein loading. B : Densitometry of protein bands from the blot shown in A . Relative expression values are normalized to those of GAPDH. C : Immunoblot of lysates (2 capsules/lane) from primary mouse LEC that grew out from dissected lens capsules (0-14 days). Lane 1, primary LEC + ILK-targeting siRNA, 7 days; lane 2, primary LEC + ILK-targeting siRNA, 14 days; lane 3, primary LEC + GFP-targeting siRNA (control), 7 days; lane 4, primary LEC + GFP-targeting siRNA. Lysates were probed for ILK to verify ILK-targeting siRNA efficiency. Fibronectin and α-SMA were monitored as indicators of EMT. An additional probe for GAPDH was used to normalize protein loading. D : Densitometry of protein bands from the blot shown in C . ILK- and GFP-siRNA-treated explants show a significant difference in protein expression after 14 days of culture (the asterisk indicates a p<0.02). Relative values are normalized to that of GAPDH.

    Article Snippet: Six μl of HiPerFect (Qiagen) was incubated with either GFP-targeting siRNA (control) or an equimolar mixture of 3 ILK-targeting siRNA constructs (#SI01076047, #SI01076054, and #SI01076061; Qiagen) in serum-free DMEM, according to the manufacturer's protocol.

    Techniques: Inhibition, Expressing, Western Blot